Gentaur® 2 × Phanta Max Master Mix - Dye Plus
2× Phanta Max Master Mix (Dye Plus) is a pre-mixed, high-fidelity PCR solution designed to deliver fast, accurate, and reliable DNA amplification. Built on Phanta Max DNA Polymerase, this master mix includes optimized buffer and two tracking dyes for direct loading onto agarose gels, streamlining your workflow.
With exceptional accuracy and enhanced processivity, this mix is ideal for routine high-fidelity PCR, cloning, mutagenesis, NGS library preparation, and more.
Key Features
- ✅ High Fidelity: Over 50× higher accuracy than Taq polymerase
- ✅ Visible Dyes Included: Direct gel loading, no separate loading buffer required
- ✅ Broad Range Template Compatibility: Works with genomic DNA, plasmids, cDNA, and complex samples
- ✅ Fast Extension Rate: ~15 sec/kb for simple templates
- ✅ Blunt-End Product: Suitable for blunt-end cloning
Technical Specifications
| Feature | Description |
|---|---|
| Enzyme | Phanta Max DNA Polymerase |
| Format | 2× Master Mix with tracking dye |
| Fidelity | >50× Taq DNA Polymerase |
| PCR Product Ends | Blunt |
| Optimal Extension Speed | 15–30 sec/kb depending on template complexity |
| Compatible Templates | Genomic DNA, plasmid DNA, cDNA, bacterial colonies |
| Loading Dye Components | Two visible dyes (Blue + Yellow) |
| Storage Temperature | –20°C |
Application Areas
- 🔬 High-Fidelity Amplification
- 🧬 DNA Cloning and Subcloning
- 🧪 Site-Directed Mutagenesis
- 🔍 Sequencing and Genotyping
- 📦 Routine PCR with Visual Gel Tracking
Protocol Summary
- Mix 2× Phanta Max Master Mix (Dye Plus) with primers and template DNA
- Add nuclease-free water to reach final volume
- Perform PCR cycling with optimized annealing and extension
- Load products directly onto agarose gel — no need for loading dye
Note: For best results, optimize annealing temperatures and primer design.
Related Resources
Why Choose Phanta Max Master Mix (Dye Plus)?
Phanta Max Master Mix combines speed, accuracy, and ease of use, making it a trusted solution in molecular biology labs worldwide. Its integrated dye system reduces handling errors and speeds up gel analysis, making it ideal for both research and diagnostic workflows.
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